Quotient Therapeutics, Inc. | Cambridge, UK Scientist, Flow Cytometry

Applied Intuition Inc.; Cambridge; On-site; GBP 50,000 - 70,000; Full time

Job summary

A biotechnology firm in Cambridge seeks a Flow Sorting Scientist to lead the flow cytometry operations in their High Throughput Laboratory. The successful candidate will optimize protocols, maintain equipment, and provide training to team members. Ideal applicants will have a degree in a related field, at least 5 years of relevant experience, and a passion for somatic genomics. Join a fast-paced team committed to advancing cutting-edge therapeutics.

Qualifications

• Minimum of 5 years' hands-on experience of flow cytometry and cell sorting.
• Deep understanding of the maintenance and set up of flow cytometry equipment.
• Experience working with multi-colour antibody panels.

Responsibilities

• Operate and troubleshoot flow cytometry and cell sorting equipment.
• Maintain Technical Procedures and SOPs for the flow cytometry platform.
• Support the design and validation of antibody panels.

Skills

Flow Cytometry

Cell Sorting

Technical Support

Cross-functional Collaboration

Molecular Biology

Excellent Communication

Education

BSc or MSc in Biochemistry, Molecular Biology or Microbiology

Tools

Laboratory Information Management Systems

Benchling

Digital Productivity Tools (Notion, Trello, Jira)

Job description

Quotient Therapeutics, Inc. is a privately held, early-stage company developing a novel genomics platform and therapeutics for diseases with a large unmet need.

Quotient Therapeutics, Inc. was founded by Flagship Pioneering, an innovation enterprise that conceives, creates, resources, and grows first-in-category life sciences companies. Flagship Pioneering has created over 100 groundbreaking companies over the past twenty years, all of which are pioneering novel and proprietary biological, industrial, and engineering approaches to solve major needs in human health and sustainability. These companies include Moderna (MRNA), Seres Therapeutics (NASDAQ: MCRB), Sana Biotechnology (SANA), Axcella Health (AXLA), Indigo Agriculture, Generate Biomedicines, and Tessera Therapeutics.

Position Summary

We are seeking a motivated, enthusiastic Flow Sorting Scientist to join our High Throughput Laboratory Operations team. The role will lead the buildout and running of our flow cytometry provision, operating and maintaining flow cytometry and cell sorting equipment. In so doing, the candidate will support both the High Throughput team and Assay Development team with experimental design and execution.

A successful candidate will be comfortable with cross-functional working, dipping in and out of ongoing projects to support delivery of prioritized work packages. The candidate will be a self-starter, able to thrive in a fast-paced, action-oriented team environment and have a clear passion for somatic genomics. They will have demonstrated evidence of agility in their scientific work and an overt attitude of positivity when facing challenges. They will be dedicated to learning and development, embrace feedback and be open to working outside their comfort zone.

Key Responsibilities

• Operate, maintain and troubleshoot flow cytometry and cell sorting equipment, ensuring optimal performance for platform activities.
• Optimize and transition effective protocols into use for High Throughput flow cytometry and cell/nuclei sorting projects.
• Maintain up to date Technical Procedures and SOPs for the Flow cytometry platform
• Provide technical support and training to other team members on the operation of flow cytometry and cell sorting equipment.
• Conduct cell sorting to isolate specific cell/nuclei populations, with high purity and viability.
• Perform sample preparation and dissociation of human tissues and cells.
• Support the design and/or adaptation of tissue dissociation and cell/nuclei isolation methods to meet project goals.
• Support the design and validation of antibody panels.
• Maintain compliance with the Human Tissue Act (HTA) and COSHH regulations.
• Work collaboratively with other team members across various functional areas.

Key Skills and Experience

• BSc or MSc degree in biochemistry, molecular biology or microbiology, or a related field.
• A minimum of 5 years' hands‑on experience of flow cytometry and cell sorting.
• Deep understanding of the maintenance and set up of flow cytometry and cell sorting equipment.
• Experience in working with multi‑colour antibody panels for flow cytometry.
• Knowledge and experience of working under HTA regulations.
• Excellent communication skills, both written and verbal.
• Use of laboratory information management systems (Benchling preferred).
• Experience in designing multi‑colour flow panels.
• Experience in spectral flow cytometry.
• Experience in the isolation and sorting of nuclei.
• Experience with optimization of tissue dissociation methods.
• Experience with next generation sequencing library preparation and other downstream molecular biology processing.
• Previous experience in a biotech or pharmaceutical industry setting.
• Experience working within a flow cytometry and cell sorting facility.
• Experience working with digital productivity tools such as Notion, Trello or Jira.

About Flagship

Flagship Pioneering is a biotechnology company that invents and builds platform companies, each with the potential for multiple products that transform human health or sustainability. Since its launch in 2000, Flagship has originated and fostered more than 100 scientific ventures, resulting in more than $90 billion in aggregate value. Many of the companies Flagship has founded have addressed humanity’s most urgent challenges: vaccinating billions of people against COVID-19, curing intractable diseases, improving human health, preempting illness, and feeding the world by improving the resiliency and sustainability of agriculture. Flagship has been recognized twice on FORTUNE’s “Change the World” list, an annual ranking of companies that have made a positive social and environmental impact through activities that are part of their core business strategies, and has been twice named to Fast Company’s annual list of the World’s Most Innovative Companies. Learn more about Flagship at www.flagshippioneering.com.

Flagship Pioneering and our ecosystem companies are committed to equal employment opportunity regardless of race, color, ancestry, religion, sex, national origin, sexual orientation, age, citizenship, marital status, disability, gender identity or Veteran status.

At Flagship, we recognize there is no perfect candidate. If you have some of the experience listed above but not all, please apply anyway. Experience comes in many forms, skills are transferable, and passion goes a long way. We are dedicated to building diverse and inclusive teams and look forward to learning more about your unique background.